Composite
hTERT+tRNA

Part:BBa_K1722010:Design

Designed by: Hao Wang   Group: iGEM15_SZU_China   (2015-09-02)


hTERT+tRNA Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both hTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital. We designed primers and amplified the gene sequences from psi-Check2 vector. Using 3A Assembly method, we constructed hTERT and tRNA in pSB1C3.


Source

Both hTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital.

References

[1] Liu Y. Research Progress in Targeting Human Telomerase Reverse Transcriptase (hTERT) Promoter in Cancer Gene Therapy, Journal of Oncology, 15(3): 150-152